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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " CD45 immunoaffinity depletion of vesicles from Jurkat T cells demonstrates that exosomes contain CD45: no evidence for a distinct exosome/HIV-1 budding pathway. | Retrovirology BioMed Central Open Access Short report CD45 immunoaffinity depletion of vesicles from Jurkat T cells demonstrates that exosomes contain CD45 no evidence for a distinct exosome HIV-1 budding pathway Lori V Coren Teresa Shatzer and David E Ott Address AIDS and Cancer Virus Program SAIC-Frederick Inc. National Cancer Institute at Frederick Frederick Maryland 21702-1201 USA Email Lori V Coren - coren@ncifcrf.gov Teresa Shatzer - tshatzer@ncifcrf.gov David E Ott - ott@ncifcrf.gov Corresponding author Published 16 July 2008 Received 11 March 2008 Retrovirology 2008 5 64 doi 10.1186 1742-4690-5-64 Accepted 16 July 2008 This article is available from http www.retrovirology.cOm content 5 1 64 2008 Coren et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The presence of relatively high levels of cellular protein contamination in density-purified virion preparations is a confounding factor in biochemical analyses of HIV and SIV produced from hematopoietic cells. A major source of this contamination is from vesicles either microvesicles or exosomes that have similar physical properties as virions. Thus these particles can not be removed by size or density fractionation. Although virions and vesicles have similar cellular protein compositions CD45 is excluded from HIV-1 yet is present in vesicles produced from hematopoietic cells. By exploiting this finding we have developed a CD45 immunoaffinity depletion procedure that removes vesicles from HIV-1 preparations. While this approach has been successfully applied to virion preparations from several different cell types some groups have concluded that exosomes from certain T cell lines specifically Jurkat do not contain CD45. If this interpretation is correct .
