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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius | BMC Plant Biology Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius Foley et al. Foley et al. BMC Plant Biology 2011 11 59 http www.biomedcentral.com 1471-2229 11 59 4 April 2011 BioMed Central Foley et al. BMC Plant Biology 2011 11 59 http www.biomedcentral.com 1471-2229 11 59 BMC Plant Biology RESEARCH ARTICLE Open Access Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius DHnnHEmIo 1 2 I in n-l inn fori1 2 AnHroiA Qr irinnc3 I cifiTi Vf Cmm4 r mr iir 2 E í ì inin5 Dor iolr ir io Qmith4 nnunud C FOley Ling Ling Udu Andrew Spriggs Leila IL jUU Danica E Goggin Penelope IVIC Smiin Craig A Atkins1 5 and Karam B Singh1 2 6 Abstract Background In legumes seed storage proteins are important for the developing seedling and are an important source of protein for humans and animals. Lupinus angustifolius L. also known as narrow-leaf lupin NLL is a grain legume crop that is gaining recognition as a potential human health food as the grain is high in protein and dietary fibre gluten-free and low in fat and starch. Results Genes encoding the seed storage proteins of NLL were characterised by sequencing cDNA clones derived from developing seeds. Four families of seed storage proteins were identified and comprised three unique a seven b two g and four S conglutins. This study added eleven new expressed storage protein genes for the species. A comparison of the deduced amino acid sequences of NLL conglutins with those available for the storage proteins of Lupinus albus L. Pisum sativum L. Medicago truncatula L. Arachis hypogaea L. and Glycine max L. permitted the analysis of a phylogenetic relationships between proteins and demonstrated in general that the strongest conservation occurred within species. In the case of 7S globulin b conglutins and 2S sulphur-rich albumin S conglutins the analysis suggests that gene duplication occurred after legume speciation. This contrasted with 11S
