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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism | Balsemão-Pires et al. BMC Plant Biology 2011 11 108 http www.biomedcentral.com 1471-2229 11 108 BMC Plant Biology RESEARCH ARTICLE Open Access The Arabidopsis translocator protein AtTSPO is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism 1.2 2.3 2 42 Emilia Balsemão-Pires Yvon Jaillais Bradley JSC Olson Leonardo R Andrade James G Umen Joanne Chory2 3 and Gilberto Sachetto-Martins1 Abstract Background The translocator protein 18 kDa TSPO previously known as the peripheral-type benzodiazepine receptor PBR is important for many cellular functions in mammals and bacteria such as steroid biosynthesis cellular respiration cell proliferation apoptosis immunomodulation transport of porphyrins and anions. Arabidopsis thaliana contains a single TSPO PBR-related gene with a 40 amino acid N-terminal extension compared to its homologs in bacteria or mammals suggesting it might be chloroplast or mitochondrial localized. Results To test if the TSPO N-terminal extension targets it to organelles we fused three potential translational start sites in the TSPO cDNA to the N-terminus of GFP AtTSPO eGFP . The location of the AtTSPO eGFP fusion protein was found to depend on the translational start position and the conditions under which plants were grown. Full-length AtTSPO eGFP fusion protein was found in the endoplasmic reticulum and in vesicles of unknown identity when plants were grown in standard conditions. However full length AtTSPO eGFP localized to chloroplasts when grown in the presence of 150 mM NaCl conditions of salt stress. In contrast when AtTSPO eGFP was truncated to the second or third start codon at amino acid position 21 or 42 the fusion protein co-localized with a mitochondrial marker in standard conditions. Using promoter GUS fusions qRT-PCR fluorescent protein tagging and chloroplast fractionation approaches we demonstrate that AtTSPO levels are regulated at the transcriptional post-transcriptional and .
