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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of differentially expressed genes associated with semigamy in Pima cotton (Gossypium barbadense L.) through comparative microarray analysis | Curtiss et al. BMC Plant Biology 2011 11 49 http www.biomedcentral.com 1471-2229 11 49 BMC Plant Biology RESEARCH ARTICLE Open Access Identification of differentially expressed genes associated with semigamy in Pima cotton Gossypium barbadense L. through comparative microarray analysis Jessica Curtiss1 Laura Rodriguez-Uribe1 J McD Stewart2 Jinfa Zhang1 Abstract Background Semigamy in cotton is a type of facultative apomixis controlled by an incompletely dominant autosomal gene Se . During semigamy the sperm and egg cells undergo cellular fusion but the sperm and egg nucleus fail to fuse in the embryo sac giving rise to diploid haploid or chimeric embryos composed of sectors of paternal and maternal origin. In this study we sought to identify differentially expressed genes related to the semigamy genotype by implementing a comparative microarray analysis of anthers and ovules between a non-semigametic Pima S-1 cotton and its doubled haploid natural isogenic mutant semigametic 57-4. Selected differentially expressed genes identified by the microarray results were then confirmed using quantitative reverse transcription PCR qRT-PCR . Results The comparative analysis between isogenic 57-4 and Pima S-1 identified 284 genes in anthers and 1 864 genes in ovules as being differentially expressed in the semigametic genotype 57-4. Based on gene functions 127 differentially expressed genes were common to both semigametic anthers and ovules with 115 being consistently differentially expressed in both tissues. Nine of those genes were selected for qRT-PCR analysis seven of which were confirmed. Furthermore several well characterized metabolic pathways including glycolysis gluconeogenesis carbon fixation in photosynthetic organisms sesquiterpenoid biosynthesis and the biosynthesis of and response to plant hormones were shown to be affected by differentially expressed genes in the semigametic tissues. Conclusion As the first report using microarray analysis several important .
