Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification and analysis of phosphorylation status of proteins in dormant terminal buds of poplar | Liu et al. BMC Plant Biology 2011 11 158 http www.biomedcentral.com 1471-2229 11 158 BMC Plant Biology RESEARCH ARTICLE Open Access Identification and analysis of phosphorylation status of proteins in dormant terminal buds of poplar Chang-Cai Liu1 2t Chang-Fu l_iu3 Hong-Xia Wang4 Zhi-Ying Shen5 Chuan-Ping Yang1 and Zhi-Gang Wei1 Abstract Background Although there has been considerable progress made towards understanding the molecular mechanisms of bud dormancy the roles of protein phosphorylation in the process of dormancy regulation in woody plants remain unclear. Results We used mass spectrometry combined with TiO2 phosphopeptide-enrichment strategies to investigate the phosphoproteome of dormant terminal buds DTBs in poplar Populus simonii X P. nigra . There were 161 unique phosphorylated sites in 161 phosphopeptides from 151 proteins 141 proteins have orthologs in Arabidopsis and 10 proteins are unique to poplar. Only 34 sites in proteins in poplar did not match well with the equivalent phosphorylation sites of their orthologs in Arabidopsis indicating that regulatory mechanisms are well conserved between poplar and Arabidopsis. Further functional classifications showed that most of these phosphoproteins were involved in binding and catalytic activity. Extraction of the phosphorylation motif using Motif-X indicated that proline-directed kinases are a major kinase group involved in protein phosphorylation in dormant poplar tissues. Conclusions This study provides evidence about the significance of protein phosphorylation during dormancy and will be useful for similar studies on other woody plants. Background Dormancy is a key feature of perennial plants. During dormancy the meristem becomes insensitive to growthpromoting signals for a period of time before it is released and growth resumes 1 2 . Bud dormancy is a critical developmental process that allows perennial plants to survive extreme seasonal variations in climate. The regulation of dormancy is a complex