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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Broader HIV-1 neutralizing antibody responses induced by envelope glycoprotein mutants based on the EIAV attenuated vaccine. | Liu et al. Retrovirology 2010 7 71 http www.retrovirology.eom content 7 1 71 RETR0VIR0L0GY RESEARCH Open Access Broader HIV-1 neutralizing antibody responses induced by envelope glycoprotein mutants based on the EIAV attenuated vaccine 1.2.3 1.4 1 1 1 1 1 1.2 Lianxing Liu Yanmin Wan 1 Lan Wu Jianping Sun Huiguang Li Haishan Li Liying Ma Yiming Shao 1 Abstract Background In order to induce a potent and cross-reactive neutralizing antibody nAb an effective envelope immunogen is crucial for many viral vaccines including the vaccine for the human immunodeficiency virus HIV . The Chinese equine infectious anemia virus EIAV attenuated vaccine has controlled the epidemic of this virus after its vaccination in over 70 million equine animals during the last 3 decades in China. Data from our past studies demonstrate that the Env protein of this vaccine plays a pivotal role in protecting horses from both homologous and heterogeneous EIAV challenges. Therefore the amino acid sequence information from the Chinese EIAV attenuated vaccine in comparison with the parental wild-type EIAV strains was applied to modify the corresponding region of the envelope glycoprotein of HIV-1 CN54. The direction of the mutations was made towards the amino acids conserved in the two EIAV vaccine strains distinguishing them from the two wild-type strains. The purpose of the modification was to enhance the immunogenicity of the HIV Env. Results The induced nAb by the modified HIV Env neutralized HIV-1 B and B C viruses at the highest titer of 1 270. Further studies showed that a single amino acid change in the C1 region accounts for the substantial enhancement in induction of anti-HIV-1 neutralizing antibodies. Conclusions This study shows that an HIV envelope modified by the information of another lentivirus vaccine induces effective broadly neutralizing antibodies. A single amino acid mutation was found to increase the immunogenicity of the HIV Env. Background Both EIAV and HIV are members of the