Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Thymic plasmacytoid dendritic cells are susceptible to productive HIV-1 infection and efficiently transfer R5 HIV-1 to thymocytes in vitro. | Evans et al. Retrovirology 2011 8 43 http www.retrovirology.eom content 8 1 43 RETR0VIR0L0GY RESEARCH Open Access Thymic plasmacytoid dendritic cells are susceptible to productive HIV-1 infection and efficiently transfer R5 HIV-1 to thymocytes in vitro -i 1ÌQCC2 A 2 n c1 I I I VC h i m i I I2 Parnoch Alzlzin 3 AiTinthiT Q ilrit i V ifJ WiA im A rin Hf 4 Ch Timn D I A in1 2 4 otiH Vdlessd A Evdls LUxSIllllll Ldl ndlllcSIl Akkild AjdlUld JUIUIIIUII Edwild vviiynt slldlUll R Lewil dld Paul U Cdmerol4 5 Abstract Background HIV-1 infection of the thymus coltributes to the defective regenerdtion dld loss of CD4 T cells il HIV-1-irifected individudls. As thymic deldrltlc cells DC dre permissive to ir ectiori by HIV-1 we exdmiled the dbility of thymic DC to el l ilfectiol of thymocytes which mdy coltribute to the overall depletiol of CD4 T cells. We compdred productive ilfectiol il isoldted humdl thymic dld blood CD11c myeloid DC mDC dld CD123 pldsmdcytoid DC pDC usilg elhdlced greel fluorescelt proteil EGFP CCR5 R5 -tropic NL AD8 dld CXCR4 X4 -tropic NL4-3 HIV-1 reporter viruses. Transfer of productive HIV-1 ilfectiol from thymic mDC dld pDC wds determiled by culturilg these DC subsets either dlole or with sorted thymocytes. Results Productive ilfectiol wds observed il both thymic pDC dld mDC followilg exposure to R5 HIV-1 dld X4 HIV-1. Thymic pDC were more frequeltly productively ilfected by both R5 dld X4 HIV-1 thdl thymic mDC p 0.03 l 6 . Thymic pDC efficieltly trdlsferred productive R5 HIV-1 ilfectiol to both CD3hi p 0.01 medl fold ilcredse of 6.5 l 6 dld CD3lc thymocytes medl fold ilcredse of 1.6 l 2 . Il compdrisol trdlsfer of productive ilfectiol by thymic mDC wds lot observed for either X4 or R5 HIV-1. Conclusions The cdpdcity of thymic pDC to efficieltly trdlsfer R5 HIV-1 to both mdture dld immdture thymocytes thdt dre otherwise refrdctory to R5 virus mdy represelt d pdthwdy to edrly ilfectiol dld impdired productiol of thymocytes dld CD4 T cells il .
