Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Strong mucosal immune responses in SIV infected macaques contribute to viral control and preserved CD4+ T-cell levels in blood and mucosal tissues. | Schultheiss et al. Retrovirology 2011 8 24 http www.retrovirology.eom content 8 1 24 RETR0VIR0L0GY RESEARCH Open Access Strong mucosal immune responses in SIV infected macaques contribute to viral control and preserved CD4 T-cell levels in blood and mucosal tissues Tina Schultheiss 1 Reiner Schulte1 2 Ulrike Sauermann1 Wiebke Ibing1 and Christiane Stahl-Hennig1 Abstract Background Since there is still no protective HIV vaccine available better insights into immune mechanism of persons effectively controlling HIV replication in the absence of any therapy should contribute to improve further vaccine designs. However little is known about the mucosal immune response of this small unique group of patients. Using the SIV-macaque-model for AIDS we had the rare opportunity to analyze 14 SIV-infected rhesus macaques durably controlling viral replication controllers . We investigated the virological and immunological profile of blood and three different mucosal tissues and compared their data to those of uninfected and animals progressing to AIDS-like disease progressors . Results Lymphocytes from blood bronchoalveolar lavage BAL and duodenal and colonic biopsies were phenotypically characterized by polychromatic flow cytometry. In controllers we observed higher levels of CD4 CD4 CCR5 and Gag-specific CD8 T-cells as well as lower immune activation in blood and all mucosal sites compared to progressors. However we could also demonstrate that immunological changes are distinct between these three mucosal sites. Intracellular cytokine staining demonstrated a significantly higher systemic and mucosal CD8 Gag-specific cellular immune response in controllers than in progressors. Most remarkable was the polyfunctional cytokine profile of CD8 lymphocytes in BAL of controllers which significantly dominated over their blood response. The overall suppression of viral replication in the controllers was confirmed by almost no detectable viral RNA in blood and all mucosal tissues .
