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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Genome-wide analysis of primary CD4+ and CD8+ T cell transcriptomes shows evidence for a network of enriched pathways associated with HIV disease. | Wu et al. Retrovirology 2011 8 18 http www.retrovirology.eom content 8 1 18 RETR0VIR0L0GY RESEARCH Open Access Genome-wide analysis of primary CD4 and CD8 T cell transcriptomes shows evidence for a network of enriched pathways associated with HIV disease 1 2 3 4 1 1 Jing Qin Wu Dominic E Dwyer Wayne B Dyer Yee Hwa Yang Bin Wang Nitin K Saksena Abstract Background HIV preferentially infects CD4 T cells and the functional impairment and numerical decline of CD4 and CD8 T cells characterize HIV disease. The numerical decline of CD4 and CD8 T cells affects the optimal ratio between the two cell types necessary for immune regulation. Therefore this work aimed to define the genomic basis of HIV interactions with the cellular transcriptome of both CD4 and CD8 T cells. Results Genome-wide transcriptomes of primary CD4 and CD8 T cells from HIV patients were analyzed at different stages of HIV disease using Illumina microarray. For each cell subset pairwise comparisons were performed and differentially expressed DE genes were identified fold change 2 and B-statistic 0 followed by quantitative PCR validation. Gene ontology GO analysis of DE genes revealed enriched categories of complement activation actin filament proteasome core and proton-transporting ATPase complex. By gene set enrichment analysis GSEA a network of enriched pathways functionally connected by mitochondria was identified in both T cell subsets as a transcriptional signature of HIV disease progression. These pathways ranged from metabolism and energy production TCA cycle and OXPHOS to mitochondria meditated cell apoptosis and cell cycle dysregulation. The most unique and significant feature of our work was that the non-progressing status in HIV long-term non-progressors was associated with MAPK WNT and AKT pathways contributing to cell survival and anti-viral responses. Conclusions These data offer new comparative insights into HIV disease progression from the aspect of HIV-host interactions at the .