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Retrovirology Research BioMed Central Open Access Accuracy estimation of foamy virus genome copying Kathleen Gärtner1, Tatiana Wiktorowicz1, Jeonghae Park2, Ayalew Mergia2, Axel Rethwilm*1 and Carsten Scheller1 Address: 1Universität Würzburg, Institut für Virologie und Immunbiologie, Versbacher Str 7, 97078, Würzburg, Germany and 2Department of Infectious Disease and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA Email: Kathleen Gärtner - Kathleen.Gaertner@gmx.de; Tatiana Wiktorowicz - Tatiana.Wiktorowicz@vim.uni-wuerzburg.de; Jeonghae Park - parkj@vetmed.ufl.edu; Ayalew Mergia - MergiaA@vetmed.ufl.edu; Axel Rethwilm* - virologie@vim.uni-wuerzburg.de; Carsten Scheller - scheller@vim.uni-wuerzburg.de * Corresponding author Published: 6 April 2009 Retrovirology 2009, 6:32 doi:10.1186/1742-4690-6-32 Received: 4 November 2008 Accepted: 6 April 2009 This article is available from: http://www.retrovirology.com/content/6/1/32 © 2009. | BioMed Central Retrovirology Open Access Accuracy estimation of foamy virus genome copying Kathleen Gartner1 Tatiana Wiktorowicz1 Jeonghae Park2 Ayalew Mergia2 Axel Rethwilm 1 and Carsten Scheller1 Address 1Universitat Wurzburg Institut fur Virologie und Immunbiologie Versbacher Str 7 97078 Wurzburg Germany and 2Department of Infectious Disease and Pathology College of Veterinary Medicine University of Florida Gainesville FL USA Email Kathleen Gartner - Kathleen.Gaertner@gmx.de Tatiana Wiktorowicz - Tatiana.Wiktorowicz@vim.uni-wuerzburg.de Jeonghae Park - parkj@vetmed.ufl.edu Ayalew Mergia - MergiaA@vetmed.ufl.edu Axel Rethwilm - virologie@vim.uni-wuerzburg.de Carsten Scheller - scheller@vim.uni-wuerzburg.de Corresponding author Published 6 April 2009 Received 4 November 2008 Retrovirology 2009 6 32 doi 10.1 186 1742-4690-6-32 Accepted 6 April 2009 This article is available from http www.retrovirology.eom content 6 1 32 2009 Gartner et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Foamy viruses FVs are the most genetically stable viruses of the retrovirus family. This is in contrast to the in vitro error rate found for recombinant FV reverse transcriptase RT . To investigate the accuracy of FV genome copying in vivo we analyzed the occurrence of mutations in HEK 293T cell culture after a single round of reverse transcription using a replication-deficient vector system. Furthermore the frequency of FV recombination by template switching TS and the cross-packaging ability of different FV strains were analyzed. Results We initially sequenced 90 000 nucleotides and detected 39 mutations corresponding to an in vivo error rate of approximately 4 X I0-4 per site per replication cycle. Surprisingly all .
