Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: IFN-gamma regulation of ICAM-1 receptors in bronchial epithelial cells: soluble ICAM–1 release inhibits human rhinovirus infection. | Journal of Inflammation BioMed Central Research IFN-gamma regulation of ICAM-I receptors in bronchial epithelial cells soluble ICAM-1 release inhibits human rhinovirus infection Suzanne C Whiteman 1 and Monica A Spiteri2 Open Access Address School of Medicine Keele University Keele UK and 2Lung Research Institute of Science Technology in Medicine Keele University and Directorate of Respiratory Medicine University Hospital of North Staffordshire UK Email Suzanne C Whiteman - suzannewhiteman@yahoo.co.uk Monica A Spiteri - monica.spiteri@uhns.nhs.uk Corresponding author Published 5 June 2008 Received 16 August 2007 Journal of Inflammation 2008 5 8 doi 10.1 186 1476-9255-5-8 Accepted 5 June 2008 This article is available from http www.journal-inflammation.cOm content 5 1 8 2008 Whiteman and Spiteri licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Intercellular adhesion molecule-1 ICAM-1 is a critical target-docking molecule on epithelial cells for 90 of human rhinovirus HRV serotypes. Two forms of ICAM-1 exist membranous mICAM-1 and soluble sICAM-1 both expressed by bronchial epithelial cells. Interferon-gamma IFN-y a crucial Th-1 immuno-regulatory mediator can modulate mICAM-1 expression however its simultaneous effects on mICAM-1 sICAM-1 levels and their consequent outcome on cell infectivity have not been previously explored. Methods Primary normal human bronchial epithelial cells were pre-stimulated with IFN-y 1 ng ml for 24 h and subsequently inoculated with HRV-14 or HRV-1 b TCID50 10 2-5 . Epithelial surface ICAM-1 expression and soluble ICAM-1 release were measured at the protein and gene level by immunofluorescence and .
