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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: HIV-1 neutralization by monoclonal antibody against conserved region 2 and patterns of epitope exposure on the surface of native viruses. | Journal of Immune Based Therapies and Vaccines BioMed Central Open Access Original research HIV-1 neutralization by monoclonal antibody against conserved region 2 and patterns of epitope exposure on the surface of native viruses Apichai Sreepian1 Jongruk Permmongkol2 Wannee Kantakamalakul1 Sontana Siritantikorn1 Nattaya Tanlieng1 and Ruengpung Sutthent 1 Address Department of Microbiology Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand and 2Faculty of Medical Technology Mahidol University Bangkok Thailand Email Apichai Sreepian - asreepian@gmail.com Jongruk Permmongkol - mtjpm@mahidol.ac.th Wannee Kantakamalakul - siwkk@mahidol.ac.th Sontana Siritantikorn - sissr@mahidol.ac.th NattayaTanlieng - dogsok@hotmail.com Ruengpung Sutthent - sirst@mahidol.ac.th Corresponding author Published 12 October 2009 Received 25 September 2008 Journal of Immune Based Therapies and Vaccines 2009 7 5 doi 10.1 186 1476-8518-7-5 AccePted 12 October 2009 This article is available from http www.jibtherapies.com content 7 1 5 2009 Sreepian et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Conserved neutralizing epitopes are considered to be a key role for eliciting broadly neutralizing antibody NAb . Previously two conserved neutralizing epitopes of HIV-1 CRF01_AE envelope were identified at amino acid 93-112 of the C1 C1E and at 218-239 of the C2 C2E regions. To access the potency of antibody directed against conserved epitopes a monoclonal antibody MAb specific to the C2E region was developed and characterized. Methods The immunogenicity of two epitopes was examined by immunizing BALB c mice with the matching synthetic peptides. One MAb C2EB5 directed against peptide C2E was generated by .
