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Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: Apoptosis of resident and inflammatory macrophages before and during the inflammatory response of the virgin bovine mammary gland. | Sladek and Rysanek Acta Veterinaria Scandinavica 2010 52 12 http www.actavetscand.eom content 52 1 12 AVS ACTAVETERINARIA SCANDINAVICA RESEARCH Open Access Apoptosis of resident and inflammatory macrophages before and during the inflammatory response of the virgin bovine mammary gland Zbysek Sladek1 2 Dusan Rysanek2 Abstract Background Macrophages may play a prominent role in defense of the bovine mammary gland and their functionality is necessary for successful eradication of bacterial pathogens. In contrast to necrosis however apoptosis has not yet been studied in macrophages from bovine mammary glands. Therefore the aim of this study was to confirm the occurrence of apoptosis in macrophages from resting heifer mammary glands and during the inflammatory response. Methods Inflammatory response was induced by phosphate buffered saline PBS and by lipopolysaccharide LPS . Resident macrophages RESMAC were obtained before and inflammatory macrophages INFMAC 24 48 72 and 168 hours after inducing inflammatory response in mammary glands of unbred heifers. Cell samples were analyzed for differential counts apoptosis and necrosis using flow cytometry. Results Populations of RESMAC and INFMAC contained monocyte-like cells and vacuolized cells. Apoptosis was detected differentially in both morphologically different types of RESMAC and INFMAC and also during initiation and resolution of the inflammatory response. In the RESMAC population approximately one-tenth of monocyte-like cells and one-third of vacuolized cells were apoptotic. In the INFMAC population obtained 24 h after PBS treatment approximately one-tenth of monocyte-like cells and almost one-quarter of vacuolized cells were apoptotic. At the same time following LPS however we observed a significantly lower percentage of apoptotic cells in the population of monocyte-like INFMAC and vacuolized INFMAC. Moreover a higher percentage of apoptotic cells in infMAC was detected during all time points after PBS in contrast to
