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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:In vivo clearance of surfactant lipids during acute pulmonary inflammation. | Respiratory Research BioMed Central Research Open Access In vivo clearance of surfactant lipids during acute pulmonary inflammation. Jaret L Malloy and Jo Rae Wright Address Address Box 3709 Department of Cell Biology Duke University Medical Center Durham NC 27710 USA Email Jaret L Malloy - j.malloy@cellbio.duke.edu Jo Rae Wright - j.wright@cellbio.duke.edu Corresponding author Published 23 July 2004 Received 30 January 2004 Respiratory Research 2004 5 8 doi 10.1186 1465-9921-5-8 Accepted 23 July 2004 This article is available from http respiratory-research.cOm content 5 1 8 2004 Malloy and Wright licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background A decrease in pulmonary surfactant has been suggested to contribute to the lung dysfunction associated with pulmonary inflammation. A number of studies have implicated surfactant clearance as a possible mechanism for altered pool sizes. The objective of the current study was to specifically investigate the mechanisms of surfactant clearance in a rodent model of acute pulmonary inflammation. Methods Inflammation was induced by intrapulmonary instillation of lipopolysaccharide LPS 100 pg kg . Lipid clearance was assessed at 18 and 72 hours post-LPS instillation by intratracheal administration of radiolabel surfactant-like liposomes 2 hours prior to isolation and analysis of inflammatory cells and type II cells. Results At both 18 and 72 hours after LPS instillation there was significantly less radioactivity recovered in the lavage fluid compared to respective control groups p 0.05 . At both time points the number of cells recovered by lavage and their associated radioactivity was greater compared to control groups p 0.01 . There was no difference in recovery of .
