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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Assessment of tissue oxygen tension: comparison of dynamic fluorescence quenching and polarographic electrode technique. | Critical Care February 2001 Vol 6 No 1 Shaw et al. Research article Assessment of tissue oxygen tension comparison of dynamic fluorescence quenching and polarographic electrode technique Andrew D Shaw Zheng Li Zach Thomas and Craig W Stevens1 Department of Critical Care Medicine The University of Texas M.D. Anderson Cancer Center Houston Texas USA department of Radiation Oncology The University of Texas M.D. Anderson Cancer Center Houston Texas USA Correspondence Dr Andrew Shaw FRCA ashaw@mdanderson.org Received 25 October 2001 Accepted 11 December 2001 Published 10 January 2002 Critical Care 2002 6 76-80 2002 Shaw et al. licensee BioMed Central Ltd Print ISSN 1364-8535 Online ISSN 1466-609X Abstract Introduction and methods Dynamic fluorescence quenching is a technique that may overcome some of the limitations associated with measurement of tissue partial oxygen tension Po2 . We compared this technique with a polarographic Eppendorf needle electrode method using a saline tonometer in which the PO2 could be controlled. We also tested the fluorescence quenching system in a rodent model of skeletal muscle ischemia-hypoxia. Results Both systems measured Po2 accurately in the tonometer and there was excellent correlation between them r2 0.99 . The polarographic system exhibited proportional bias that was not evident with the fluorescence method. In vivo the fluorescence quenching technique provided a readily recordable signal that varied as expected. Discussion Measurement of tissue Po2 using fluorescence quenching is at least as accurate as measurement using the Eppendorf needle electrode in vitro and may prove useful in vivo for assessment of tissue oxygenation. Keywords clinical measurement methodology fiberoptic measurement fluorescence quenching ischemia Stern-Volmer tissue oxygenation Introduction Accurate measurement of Po2 in biologic tissues has been of interest to both researchers and clinicians for many years 1 . For basic scientists measurement of PO2 .
