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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Characterization of a novel and spontaneous mouse model of inflammatory arthritis. | Adipue et al. Arthritis Research Therapy 2011 13 R114 http arthritis-research.eom content 13 4 R114 RESEARCH ARTICLE Open Access Characterization of a novel and spontaneous mouse model of inflammatory arthritis 111 1 111 Iris A Adipue Joel T Wilcox Cody King Carolyn AY Rice Katherine M Shaum Cory M Suard Elri ten Brink Stephen D Miller2 and Eileen J McMahon1 Abstract Introduction Mouse models of rheumatoid arthritis RA have proven critical for identifying genetic and cellular mechanisms of the disease. Upon discovering mice in our breeding colony that had spontaneously developed inflamed joints reminiscent of RA we established the novel IIJ inherited inflamed joints strain. The purpose of this study was to characterize the histopathological clinical genetic and immunological properties of the disease. Methods To begin the IIJ strain an arthritic male mouse was crossed with SJL J females. Inheritance of the phenotype was then tracked by intercrossing backcrossing and outcrossing to other inbred strains. The histopathology of the joints and extraarticular organ systems was examined. Serum cytokines and immunoglobulins Igs were measured by ELISA and cytometric bead array. Transfer experiments tested whether disease could be mediated by serum alone. Finally the cellular joint infiltrate and the composition of secondary lymphoid organs were examined by immunohistochemistry and flow cytometry. Results After nine generations of intercrossing the total incidence of arthritis was 33 304 of 932 mice with females being affected more than males 38 vs. 28 P 0.001 . Swelling most notably in the large distal joints typically became evident at an early age mean age of 52 days . In addition to the joint pathology which included bone and cartilage erosion synovial hyperproliferation and a robust cellular infiltration of mostly Gr-1 neutrophils there was also evidence of systemic inflammation. IL-6 was elevated in the sera of recently arthritic mice and extraarticular inflammation .