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A water soluble glucan isolated from hot aqueous extract of fruit bodies of an edible hybrid mushroom Pfle1r of Pleurotus florida and Lentinula edodes showed macrophages, splenocytes, and thymocytes activation. The glucan consists of terminal, (1→3,6)-linked, and (1→6)-linked β-D-glucopyranosyl moieties in a molar ratio of nearly 1:1:3. On the basis of acid hydrolysis, methylation, periodate oxidation study, and NMR studies (1H, 13C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC), the structure of the repeating unit of the glucan was established as:. | Accepted Manuscript Structural characterization and study of immunoenhancing properties of a glucan isolated from a hybrid mushroom of Pleurotus florida andLentinula edodes Praloy K. Maji Ipsita K. Sen Birendra Behera Tapas K. Maiti Pijush Mallick Samir R. Sikdar Syed S. Islam PII DOI Reference S0008-6215 12 00277-7 http dx.doi.org 10.1016 j.carres.2012.06.017 CAR 6220 To appear in Carbohydrate Research Received Date Revised Date Accepted Date 7 June 2012 20 June 2012 21 June 2012 Please cite this article as Maji P.K. Sen I.K. Behera B. Maiti T.K. Mallick P. Sikdar S.R. Islam S.S. Structural characterization and study of immunoenhancing properties of a glucan isolated from a hybrid mushroom of Pleurotus florida andLentinula edodes Carbohydrate Research 2012 doi http dx.doi.org 10.1016 j.carres. 2012.06.017 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting typesetting and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 Structural characterization and study of immunoenhancing properties of a glucan isolated from a hybrid mushroom of Pleurotus florida and Lentinula edodes TJ-pol AV A f n i i a Tn ci t Q Q a T i von r ro T ế ịlế VQ lortoc Tk A fn i t ib Praloy K. Iviaji Ipsita K. sen Birendra Behera Tapas K. Iviaiti Samir R. Sikdarc Syed s. Islama Department of Chemistry and Chemical Technology Vidyasagar University Midnapore 721102 West Bengal India bDepartment of Biotechnology Indian Institute of Technology IIT Kharagpur .
