Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis. | Takei et al. Arthritis Research Therapy 2011 13 R52 http arthritis-research.eom content 13 2 R52 RESEARCH ARTICLE Open Access Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis Satoko Takei Tomoaki Hoshino Kazuko Matsunaga Yuki Sakazaki Masanori Sawada Hanako Oda Shin-ichi Takenaka Haruki Imaoka Takashi Kinoshita Seiyo Honda Hiroaki Ida Taka-aki Fukuda and Hisamichi Aizawa Abstract Introduction There has been no report in the literature of a soluble form of interleukin IL -18 receptor a IL-18Ra . In this study we evaluated the levels and characteristics of soluble IL-18Ra sIL-18Ra in the sera of patients with rheumatoid arthritis RA and compared these results to control populations. Methods The sIL-18Ra complex was isolated from pooled human blood serum using an anti-IL-18Ra monoclonal antibody affinity column. The purified sIL-18Ra was then examined using Western blot analysis and used in experiments to evaluate the effects on an IL-18-responsive natural killer NK human cell line NK0. An enzyme-linked immunosorbent assay was developed and sera from 145 patients with RA 6 patients with adult-onset Still s disease 31 patients with osteoarthritis OA 39 patients with systemic lupus erythematosus SLE and 67 controls were tested along with levels of immunoglobulin M rheumatoid factor anticyclic citrullinated peptide antibody IL-18 IL-13 and interferon IFN -y. Area under the receiver operating characteristic curve ROC-AUC analysis was used to evaluate the diagnostic utility of the sIL-18Ra complex. Results The isolated sIL-18Ra complex can be associated with IL-18 and the soluble form of the IL-18RP chain. The sIL-18Ra complex bound to the surface to the NK0 cell line antagonized the stimulatory effects of IL-18 and IL-2 on the NK0 cell line and inhibited IFN-y production by the cells. The serum levels of sIL-18Ra complex in RA 186.0 33.5 ng mL n 145 and adult-onset Still s disease 98.2 8.9 ng mL n 6 were significantly P 0.001 higher .