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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Decreased frequency and activated phenotype of blood CD27 IgD IgM B lymphocytes is a permanent abnormality in systemic lupus erythematosus patients. | Rodriguez-Bayona et al. Arthritis Research Therapy 2010 12 R108 http arthritis-research.eom content 12 3 R108 RESEARCH ARTICLE Open Access Decreased frequency and activated phenotype of blood CD27 IgD IgM B lymphocytes is a permanent abnormality in systemic lupus erythematosus patients Beatriz Rodriguez-Bayona1 Ana Ramos-Amaya1 José J Perez-Venegas2 Carmen Rodríguez 3 and José A Brieva 3 Abstract Introduction Systemic lupus erythematosus SLE is characterized by B cell hyper-activation and auto-reactivity resulting in pathogenic auto-antibody generation. The phenotypic analysis of blood B cell subsets can be used to understand these alterations. Methods The combined detection of CD19 CD27 and IgD or IgM by flow cytometry FC analysis delineates five well-defined blood B cell-subsets naive switched S memory double negative DN memory and CD27 IgD IgM nonswitched memory B lymphocytes and plasma cells PCs . This phenotypic study was performed in 69 consecutive SLE patients and 31 healthy controls. Results SLE patients exhibited several abnormalities in the distribution of these B cell subsets including elevated levels of DN memory B cells and PCs and decreased CD27 IgD IgM B cells. Active SLE patients also showed decreased presence of S memory B cells and increased proportions of naive B lymphocytes. Nevertheless when the patients in remission who did not require treatment were studied separately the only remaining abnormality was a reduction of the CD27 IgD IgM B cell-subset detectable in most of these patients. The level of reduction of CD27 IgD IgM B cells was associated with elevated values of serum SLE auto-antibodies. Further analysis of this latter B cell-subset specifically showed increased expression of CD80 CD86 CD95 9G4 idiotype and functional CXCR3 and CXCR4. Conclusions The presence of a reduced blood CD27 IgD IgM B cell-subset exhibiting an activated state and enriched for auto-reactivity is a consistent B cell abnormality in SLE. These findings suggest .