Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Characterization of monocyte/macrophage subsets in the skin and peripheral blood derived from patients with systemic sclerosis. | Higashi-Kuwata et al. Arthritis Research Therapy 2010 12 R128 http arthritis-research.eom content 12 4 R128 RESEARCH ARTICLE Open Access Characterization of monocyte macrophage subsets in the skin and peripheral blood derived from patients with systemic sclerosis Nobuyo Higashi-Kuwata 1 Masatoshi Jinnin 1 Takamitsu Makino1 Satoshi Fukushima1 Yuji Inoue1 Faith C Muchemwa1 Yuji Yonemura2 Yoshihiro Komohara3 Motohiro Takeya3 Hiroaki Mitsuya4 5 and Hironobu Ihn1 Abstract Introduction Recent accumulating evidence indicates a crucial involvement of macrophage lineage in the pathogenesis of systemic sclerosis SSc . To analyze the assembly of the monocyte macrophage population we evaluated the expression of CD163 and CD204 and various activated macrophage markers in the inflammatory cells of the skin and in the peripheral blood mononuclear cells PBMCs derived from patients with SSc. Methods Skin biopsy specimens from 6 healthy controls and 10 SSc patients 7 limited cutaneous SSc and 3 diffuse cutaneous SSc were analyzed by immunohistochemistry using monoclonal antibody against CD68 pan-macrophage marker CD163 and CD204. Surface and or intracellular protein expression of CD14 marker for monocyte lineage CD163 and CD204 was analysed by flow cytometry in PBMCs from 16 healthy controls and 41 SSc patients 26 limited cutaneous SSc and 15 diffuse cutaneous SSc . Statistical analysis was carried out using Mann-Whitney U test for comparison of means. Results In the skin from SSc patients the number of CD163 cells or CD204 cells between the collagen fibers was significantly larger than that in healthy controls. Flow cytometry showed that the population of CD14 cells was significantly greater in PBMCs from SSc patients than that in healthy controls. Further analysis of CD14 cells in SSc patients revealed higher expression of CD163 and the presence of two unique peaks in the CD204 histogram. Additionally we found that the CD163 cells belong to CD14brightCD204 population. Conclusions
