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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Collagen V-induced nasal tolerance downregulates pulmonary collagen mRNA gene and TGF-beta expression in experimental systemic sclerosis. | Velosa et al. Respiratory Research 2010 11 1 http respiratory-research.eom content 11 1 1 RESPIRATORY RESEARCH RESEARCH Open Access Collagen V-induced nasal tolerance downregulates pulmonary collagen mRNA gene and TGF-beta expression in experimental systemic sclerosis Ana Paula P Velosa1 Walcy R Teodoro1 Daniel M dos Anjos1 Renata Konno1 Cristiane C Oliveira1 Maria LH Katayama2 Edwin R Parra3 Vera L Capelozzi3 Natalino H Yoshinari1 Abstract Background The purpose of this study was to evaluate collagen deposition mRNA collagen synthesis and TGF-beta expression in the lung tissue in an experimental model of scleroderma after collagen V-induced nasal tolerance. Methods Female New Zealand rabbits N 12 were immunized with 1 mg ml of collagen V in Freund s adjuvant IM . After 150 days six immunized animals were tolerated by nasal administration of collagen V 25 pg day IM-TOL daily for 60 days. The collagen content was determined by morphometry and mRNA expressions of types I III and V collagen were determined by Real-time PCR. The TGF-beta expression was evaluated by immunostaining and quantified by point counting methods. To statistic analysis ANOVA with Bonferroni test were employed for multiple comparison when appropriate and the level of significance was determined to be p 0.05. Results IM-TOL when compared to IM showed significant reduction in total collagen content around the vessels 0.371 0.118 vs. 0.874 0.282 p 0.001 bronchioles 0.294 0.139 vs. 0.646 0.172 p 0.001 and in the septal interstitium 0.027 0.014 vs. 0.067 0.039 p 0.026 . The lung tissue of IM-TOL when compared to IM showed decreased immunostaining of types I III and V collagen reduced mRNA expression of types I 0.10 0.07 vs. 1.0 0.528 p 0.002 and V 1.12 0.42 vs. 4.74 2.25 p 0.009 collagen in addition to decreased TGF-beta expression p 0.0001 . Conclusions Collagen V-induced nasal tolerance in the experimental model of SSc regulated the pulmonary remodeling process inhibiting collagen deposition and .
