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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation. | Vitadello et al. Arthritis Research Therapy 2010 12 R52 http arthritis-research.eom content 12 2 R52 RESEARCH ARTICLE Open Access Myofiber stress-response in myositis parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation 1 f 2f 2.3 2 3 Maurizio Vitadello Andrea Doria Elena Tarricone Anna Ghirardello Luisa Gorza Abstract Introduction The endoplasmic reticulum ER stress-response evoked in mice by the overexpression of class I major histocompatibility complex antigen MHC-I was proposed as a major mechanism responsible for skeletal muscle damage and dysfunction in autoimmune myositis. The present study was undertaken to characterize in more detail the ER stress-response occurring in myofibers of patients with inflammatory myopathies focusing on the expression and distribution of Grp94 calreticulin and Grp75 three ER chaperones involved in immunomodulation. Methods Muscle biopsies were obtained from seven healthy subjects and 29 myositis patients who were subdivided into groups based on the morphological evidence of inflammation and or sarcolemmal immunoreactivity for MHC-I. Biopsies were analyzed by means of immunohistochemistry and western blot using anti-Grp94 anti-calreticulin and anti-Grp75 specific antibodies. Parallel analyses on these ER chaperones were conducted in rabbit and or murine skeletal muscle after experimental induction of regeneration or systemic inflammation. Results Upregulation of Grp94 characterized regenerating myofibers of myositis patients P 0.03 compared with values detected in biopsies without signs of muscle regeneration and developing and regenerating myofibers of mouse muscles. Conversely levels of calreticulin and Grp75 increased about fourfold and twofold respectively in patient biopsies positive for sarcolemmal MHC-I immunoreactivity compared with healthy subjects and patients negative for both inflammation and MHC-I labeling P 0.005 . Differently from calreticulin the Grp75
