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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Natural killer cells control a T-helper 1 response in patients with Behçet's disease. | Yamaguchi et al. Arthritis Research Therapy 2010 12 R80 http arthritis-research.eom content 12 3 R80 RESEARCH ARTICLE Open Access Natu ral killer cells control a T-helper 1 response in patients with Behcet s disease Yukie Yamaguchi 1 2 Hayato Takahashi3 Takashi Satoh1 Yuka Okazaki1 Nobuhisa Mizuki4 Kazuo Takahashi2 Zenro Ikezawa2 and Masataka Kuwana 1 Abstract Introduction Behqet s disease BD is a multisystem inflammatory disorder in which a T-helper 1 Th1 -polarized immune response plays a major role in the pathogenic process. We evaluated the regulatory role of natural killer NK cells in Th1-biased immune responses in patients with BD. Methods We studied 47 patients with BD including 10 with active disease aBD and 37 with inactive disease iBD and 29 healthy controls. The activation status and cytotoxic activity of NK cells were examined by flow cytometry. The levels of mRNAs for immune modulatory and cytotoxic molecules in NK cells were determined by quantitative PCR. The IL-12 signal strength in NK cells was determined by assessing the phosphorylation state of its downstream component signal transducer and activator of transduction 4 by immunoblotting. Finally NK cells ability to modulate the Th1 response was evaluated by co-culturing NK cells and T cells without cell contact. Results CD69 -activated NK cells were significantly increased in aBD compared with iBD or control samples although their cytotoxic activities were similar. The iBD NK cells showed downregulated IL-12 receptor P2 mRNA levels compared with aBD or control NK cells. The increased IL-13 expression was detected in a subset of BD patients most of them had iBD. The IL-13 expression level in iBD patients was significantly higher than the level in controls but was not statistically different compared with the level in aBD patients. The gene expression profile in iBD patients was consistent with the NK type 2 phenotype and the shift to NK type 2 was associated with disease remission. NK cells from .
