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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: A Rac1 inhibitory peptide suppresses antibody production and paw swelling in the murine collagen-induced arthritis model of rheumatoid arthritis. | Abreu et al Arthritis Research Therapy 2010 12 R2 http arthritis-research.eom content 12 1 R2 RESEARCH ARTICLE Open Access A Rpcl inhibitory peptide suppresses antibody production and paw swelling in the murine collagen-induced arthritis model of rheumatoid arthritis Joana RF Abreu Wendy Dontje1 Sarah Krausz1 Daphne de Launay1 Paula B van Hennik2 Anne-Marieke van Stalborch2 Jean-Paul ten Klooster2 Marjolein E Sanders1 Kris A Reedquist1 Margriet J Vervoordeldonk1 3 Peter L Hordijk2 and Paul PTak 1 Abstract Introduction The Rho family GTPase Rac1 regulates cytoskeletal rearrangements crucial for the recruitment extravasation and activation of leukocytes at sites of inflammation. Rac1 signaling also promotes the activation and survival of lymphocytes and osteoclasts. Therefore we assessed the ability of a cell-permeable Rac1 carboxy-terminal inhibitory peptide to modulate disease in mice with collagen-induced arthritis CIA . Methods CIA was induced in DBA 1 mice and in either early or chronic disease mice were treated three times per week by intraperitoneal injection with control peptide or Rac1 inhibitory peptide. Effects on disease progression were assessed by measurement of paw swelling. Inflammation and joint destruction were examined by histology and radiology. Serum levels of anti-collagen type II antibodies were measured by enzyme-linked immunosorbent assay. T-cell phenotypes and activation were assessed by fluorescence-activated cell sorting analysis. Results were analyzed using Mann-Whitney U and unpaired Student t tests. Results Treatment of mice with Rac1 inhibitory peptide resulted in a decrease in paw swelling in early disease and to a lesser extent in more chronic arthritis. Of interest while joint destruction was unaffected by Rac1 inhibitory peptide anti-collagen type II antibody production was significantly diminished in treated mice in both early and chronic arthritis. Ex vivo Rac1 inhibitory peptide suppressed T-cell receptor CD28-dependent .
