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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Open Access No evidence of altered alveolar macrophage polarization, but reduced expression of TLR2, in bronchoalveolar lavage cells in sarcoidosis. | Wikén et al. Respiratory Research 2010 11 121 http respiratory-research.eom content 11 1 121 RESPIRATORY RESEARCH RESEARCH Open Access No evidence of altered alveolar macrophage polarization but reduced expression of TLR2 in bronchoalveolar lavage cells in sarcoidosis 1 1.2 1 1 1 1 Maria Wikén Farah Idali Muntasir Abo Al Hayja Johan Grunewald Anders Eklund Jan Wahlstrom Abstract Background Sarcoidosis is a granulomatous inflammatory disease possibly of infectious aetiology. We aimed to investigate whether the degree of functional polarization of alveolar macrophages AMs or Toll-like receptor TLR expression is associated with sarcoidosis or with distinct clinical manifestations of this disease. Methods Total BAL cells cultured four or 24 h in medium or stimulated 24 h with LPS from 14 patients and six healthy subjects sorted AMs from 22 patients Lofgren s syndrome n 11 and 11 healthy subjects and sorted CD4 T cells from 26 patients Lofgren s syndrome n 13 and seven healthy subjects were included. Using realtime PCR the relative gene expression of IL-10 IL-12p35 IL-12p40 IL-23p19 CCR2 CCR7 iNOS CXCL10 CXCL11 CXCL16 CCL18 CCL20 CD80 and CD86 and innate immune receptors TLR2 TLR4 and TLR9 was quantified in sorted AMs and for selected genes in total BAL cells while IL-17A was quantified in T cells. Results We did not find evidence of a difference with regard to alveolar macrophage M1 M2 polarization between sarcoidosis patients and healthy controls. TLR2 gene expression was significantly lower in sorted AMs from patients particular in Lofgren s patients. CCL18 gene expression in AMs was significantly higher in patients compared to controls. Additionally the IL-17A expression was lower in Lofgren s patients CD4 T cells. Conclusions Overall there was no evidence for alveolar macrophage polarization in sarcoidosis. However there was a reduced TLR2 mRNA expression in patients with Lofgren s syndrome which may be of relevance for macrophage interactions with a postulated .
