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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"The TGF-beta-Pseudoreceptor BAMBI is strongly expressed in COPD lungs and regulated by nontypeable Haemophilus influenzae. | Dromann et al. Respiratory Research 2010 11 67 http respiratory-research.eom content 11 1 67 RESPIRATORY RESEARCH RESEARCH Open Access The TGF-beta-Pseudoreceptor BAMBI is strongly expressed in COPD lungs and regulated by nontypeable Haemophilus influenzae Daniel Dromann 1 Jan Rupp1 2 Kristina Rohmann1 Sinia Osbahr1 Artur J Ulmer3 Sebastian Marwitz4 Kristina Roschmann3 Mahdi Abdullah4 Holger Schultz4 Ekkehard Vollmer4 Peter Zabel1 5 Klaus Dalhoff1 and Torsten Goldmann4 Abstract Background Nontypeable Haemophilus influenzae NTHI may play a role as an infectious trigger in the pathogenesis of chronic obstructive pulmonary disease COPD . Few data are available regarding the influence of acute and persistent infection on tissue remodelling and repair factors such as transforming growth factor TGF -p. Methods NTHI infection in lung tissues obtained from COPD patients and controls was studied in vivo and using an in vitro model. Infection experiments were performed with two different clinical isolates. Detection of NTHI was done using in situ hybridization ISH in unstimulated and in in vitro infected lung tissue. For characterization of TGF-P signaling molecules a transcriptome array was performed. Expression of the TGF-pseudoreceptor BMP and Activin Membrane-bound Inhibitor BAMBI was analyzed using immunohistochemistry IHC ISH and PCR. CXC chemokine ligand CXCL -8 tumor necrosis factor TNF -a and TGF-P expression were evaluated in lung tissue and cell culture using ELISA. Results In 38 of COPD patients infection with NTHI was detected in vivo in contrast to 0 of controls p 0.05 . Transcriptome arrays showed no significant changes of TGF-P receptors 1 and 2 and Smad-3 expression whereas a strong expression of BAMBI with upregulation after in vitro infection of COPD lung tissue was demonstrated. BAMBI was expressed ubiquitously on alveolar macrophages AM and to a lesser degree on alveolar epithelial cells AEC . Measurement of cytokine concentrations in lung tissue .
