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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Respiratory syncytial virus infection reduces lung inflammation and fibrosis in mice exposed to vanadium. | Turpin et al. Respiratory Research 2010 11 20 http respiratory-research.eom content 11 1 20 RESPIRATORY RESEARCH RESEARCH Open Access Respiratory syncytial virus infection reduces lung inflammation and fibrosis in mice exposed to vanadium pentoxide 1 1 1.2.3 1 1 Elizabeth A Turpin Aurita Antao-Menezes Mark F Cesta James B Mangum Duncan G Wallace Edilberto Bermudez1 James C Bonner1 3 Abstract Background Vanadium pentoxide V2O5 exposure is a cause of occupational bronchitis and airway fibrosis. Respiratory syncytial virus RSV is a ubiquitous pathogen that causes airway inflammation. It is unknown whether individuals with pre-existing respiratory viral infection are susceptible to V2O5-induced bronchitis. We hypothesized that respiratory viral infection will exacerbate vanadium-induced lung fibrosis. Methods In this study we investigated the effect of RSV pre- or post-exposure to V2O5 in male AKR mice. Mice were pre-exposed by intranasal aspiration to RSV or media vehicle prior to intranasal aspiration of V2O5 or saline vehicle at day 1 or day 7. A parallel group of mice were treated first with V2O5 or saline vehicle at day 1 and day 7 then post-exposed to RSV or media vehicle at day 8. Results V2O5-induced airway inflammation and fibrosis were decreased by RSV pre- or post-exposure. Real time quantitative RT-PCR showed that V2O5 significantly increased lung mRNAs encoding pro-fibrogenic growth factors TGF-P1 CTGF PDGF-C and collagen Col1A2 but also increased mRNAs encoding anti-fibrogenic type I interferons IFN-a -b and IFN-inducible chemokines CXCL9 and CXCL10 . RSV pre- or post-exposure caused a significantly reduced mRNAs of pro-fibrogenic growth factors and collagen yet reduced RNA levels of anti-fibrogenic interferons and CXC chemokines. Conclusions Collectively these data suggest that RSV infection reduces the severity of V2O5-induced fibrosis by suppressing growth factors and collagen genes. However RSV suppression of V2O5-induced IFNs and IFN-inducible .
