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Smear microscopy and culture forms the backbone of tuberculosis (TB) laboratory investigations in tertiary healthcare facilities which have a large number of cases and financial constraints. The present study aimed to re-evaluate the efficiency of smear microscopy and culture on Lowenstein Jensen (LJ) medium for acid fast bacilli (AFB) isolated from patients with pulmonary tuberculosis. 210 samples were processed for detection of AFB by Ziehl Neelsen (ZN) staining. Concentration method of N-acetly-L-cystein-NaOH was used and the samples were isolated on LJ medium. AFB was seen in 168 (80.0%) primary smear samples. The primary smear missed 5 (11.9%) samples that were. | elSSN 09748369 Diagnosis of pulmonary tuberculosis by smear microscopy and . culture in a tertiary health care facility Biology and Medicine SI Khatib MT Williamson R Singh JM Joshi Accepted 28th Feb 2012 Published 6th Apr 2012 Volume 4 Issue 1 Page 32-36 2012 Research Article Biology and Medicine 4 1 32-36 2012 www.biolmedonline.com Diagnosis of pulmonary tuberculosis by smear microscopy and culture in a tertiary health care facility SI Khatib1 3 MT Williamson1 R Singh2 JM Joshi2 1 Department of Microbiology 2 Department of Pulmonary Medicine TN Medical College and BYL Nair Charitable Hospital Mumbai India. 3 Department of Biology Faculty of Science Jazan University Jazan Kingdom of Saudi Arabia. Corresponding Author kismail@jazanu.edu.sa sayeedkhatib@hotmail.com Abstract Smear microscopy and culture forms the backbone of tuberculosis TB laboratory investigations in tertiary healthcare facilities which have a large number of cases and financial constraints. The present study aimed to re-evaluate the efficiency of smear microscopy and culture on Lowenstein Jensen LJ medium for acid fast bacilli AFB isolated from patients with pulmonary tuberculosis. 210 samples were processed for detection of AFB by Ziehl Neelsen ZN staining. Concentration method of N-acetly-L-cystein-NaOH was used and the samples were isolated on LJ medium. AFB was seen in 168 80.0 primary smear samples. The primary smear missed 5 11.9 samples that were detected by secondary smear Sensitivity 93.45 Specificity 88.10 Positive predictive value PPV 96.91 and Negative Predictive value NPV 77.08 . Growth was observed in 155 83.30 samples Sensitivity 95.39 Specificity 70.59 PPV 93.55 and NPV 77.42 . The values were statistically significant. The present study reconfirms the efficiency of conventional ZN staining method and culture on LJ medium in the detection of AFB in sputum samples of patients with pulmonary tuberculosis. Keywords Acid fast bacilli culture Lowenstein Jensen medium pulmonary .
