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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Porphyrin-magnetite nanoconjugates for biological imaging | Nowostawska et al. Journal of Nanobiotechnology 2011 9 13 JOURNAL OF NANOBIOTECHNOLOGY http www.jnanobiotechnology.eom content 9 1 13 RESEARCH Open Access Porphyrin-magnetite nanoconjugates for biological imaging 12 A z- 3 1 2 2 1 Malgorzata Nowostawska Serena A Corr Stephen J Byrne Jennifer Conroy Yuri Volkov and Yurii K Gun ko Abstract Background The use of silica coated magnetic nanoparticles as contrast agents has resulted in the production of highly stable non-toxic solutions that can be manipulated via an external magnetic field. As a result the interaction of these nanocomposites with cells is of vital importance in understanding their behaviour and biocompatibility. Here we report the preparation characterisation and potential application of new two-in-one magnetic fluorescent nanocomposites composed of silica-coated magnetite nanoparticles covalently linked to a porphyrin moiety. Method The experiments were performed by administering porphyrin functionalised silica-coated magnetite nanoparticles to THP-1 cells a human acute monocytic leukaemia cell line. Cells were cultured in RPMI 1640 medium with 25 mM HEPES supplemented with heat-inactivated foetal bovine serum FBS . Results We have synthesised characterised and analysed in vitro a new multimodal magnetic and fluorescent porphyrin magnetic nanoparticle composite PMNC . Initial co-incubation experiments performed with THP-1 macrophage cells were promising however the PMNC photobleached under confocal microscopy study. P-mercaptoethanol P-ME was employed to counteract this problem and resulted not only in enhanced fluorescence emission but also allowed for elongated imaging and increased exposure times of the PMNC in a cellular environment. Conclusion Our experiments have demonstrated that P-ME visibly enhances the emission intensity. No deleterious effects to the cells were witnessed upon co-incubation with P-ME alone and no increases in background fluorescence were recorded. These results should present
