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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging | Hou et al. Journal of Nanobiotechnology 2010 8 25 http www.jnanobiotechnology.eom content 8 1 25 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging Yong Hou1 2t Yingxun Liu1 Zhongping Chen1 Ning Gu1 Jinke Wang1 2 Abstract Background In recent years near-infrared fluorescence NIRF -labeled iron nanoparticles have been synthesized and applied in a number of applications including the labeling of human cells for monitoring the engraftment process imaging tumors sensoring the in vivo molecular environment surrounding nanoparticles and tracing their in vivo biodistribution. These studies demonstrate that NIRF-labeled iron nanoparticles provide an efficient probe for cell labeling. Furthermore the in vivo imaging studies show excellent performance of the NIR fluorophores. However there is a limited selection of NIRF-labeled iron nanoparticles with an optimal wavelength for imaging around 800 nm where tissue autofluorescence is minimal. Therefore it is necessary to develop additional alternative NIRF-labeled iron nanoparticles for application in this area. Results This study manufactured 12-nm DMSA-coated Fe3O4 nanoparticles labeled with a near-infrared fluorophore IRDye800CW excitation emission 774 789 nm to investigate their applicability in cell labeling and in vivo imaging. The mouse macrophage RAW264.7 was labeled with IRDye800CW-labeled Fe3O4 nanoparticles at concentrations of 20 30 40 50 60 80 and 100 pg ml for 24 h. The results revealed that the cells were efficiently labeled by the nanoparticles without any significant effect on cell viability. The nanoparticles were injected into the mouse via the tail vein at dosages of 2 or 5 mg kg body weight and the mouse was discontinuously imaged for 24 h. The results demonstrated that the nanoparticles gradually accumulated in liver and kidney regions following injection reaching maximum concentrations at 6 h .