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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A high sensitivity assay for the inflammatory marker C-Reactive protein employing acoustic biosensing | Journal of Nanobiotechnology BioMed Central Research Open Access A high sensitivity assay for the inflammatory marker C-Reactive protein employing acoustic biosensing Jeffrey D McBride and Matthew A Cooper Address Akubio Ltd. 181 Cambridge Science Park Cambridge CB4 0GJ UK Email Jeffrey D McBride - jeffreymcb@googlemail.com Matthew A Cooper - mc221@cam.ac.uk Corresponding author Published 29 April 2008 Received 6 September 2007 _k 1- j_.izviirtz n-7-7- irrzr Accepted 29 April 2008 Journal of Nanobiotechnology 2008 6 5 doi 10.1186 1477-3 155-6-5 This article is available from http www.jnanobiotechnology.cOm content 6 1 5 2008 McBride and Cooper licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract C-Reactive Protein CRP is an acute phase reactant routinely used as a biomarker to assess either infection or inflammatory processes such as autoimmune diseases. CRP also has demonstrated utility as a predictive marker of future risk of cardiovascular disease. A new method of immunoassay for the detection of C-Reactive Protein has been developed using Resonant Acoustic Profiling RAP with comparable sensitivity to a high sensitivity CRP ELISA hsCRP but with considerable time efficiency 12 minutes turnaround time to result . In one method standard solutions of CRP 0 to 231 ng mL or diluted spiked horse serum sample are injected through two sensor channels of a RAP biosensor. One contains a surface with sheep antibody to CRP the other a control surface containing purified Sheep IgG. At the end of a 5-minute injection the initial rate of change in resonant frequency was proportional to CRP concentration. The initial rates of a second sandwich step of anti-CRP binding were also proportional to the sample CRP concentration and .
