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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: MicroRNA expression profiles in human cancer cells after ionizing radiation. | Niemoeller et al. Radiation Oncology 2011 6 29 http www.ro-journal.eom content 6 1 29 RADIATION ONCOLOGY RESEARCH Open Access MicroRNA expression profiles in human cancer cells after ionizing radiation Olivier M Niemoeller Maximilian Niyazi Stefanie Corradini Franz Zehentmayr Minglun Li Kirsten Lauber and Claus Belka Abstract Introduction MicroRNAs are regulators of central cellular processes and are implicated in the pathogenesis and prognosis of human cancers. MicroRNAs also modulate responses to anti-cancer therapy. In the context of radiation oncology microRNAs were found to modulate cell death and proliferation after irradiation. However changes in microRNA expression profiles in response to irradiation have not been comprehensively analyzed so far. The present study s intend is to present a broad screen of changes in microRNA expression following irradiation of different malignant cell lines. Materials and methods 1100 microRNAs Sanger miRBase release version 14.0 were analyzed in six malignant cell lines following irradiation with clinically relevant doses of 2.0 Gy. MicroRNA levels 6 hours after irradiation were compared to microRNA levels in non-irradiated cells using the Geniom Biochip MPEA homo sapiens . Results Hierarchical clustering analysis revealed a pattern which significantly p 0.014 discerned irradiated from non-irradiated cells. The expression levels of a number of microRNAs known to be involved in the regulation of cellular processes like apoptosis proliferation invasion local immune response and radioresistance e. g. miR-1285 miR-24-1 miR-151-5p let-7i displayed 2 - 3-fold changes after irradiation. Moreover several microRNAs previously not known to be radiation-responsive were discovered. Conclusion Ionizing radiation induced significant changes in microRNA expression profiles in 3 glioma and 3 squamous cell carcinoma cell lines. The functional relevance of these changes is not addressed but should by analyzed by future work especially .
