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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Exogenous sphingomyelinase increases collagen and sulphated glycosaminoglycan production by primary articular chondrocytes: an in vitro study. | Available online http arthritis-research.eom content 8 4 R89 Research article Exogenous sphingomyelinase increases collagen and sulphated glycosaminoglycan production by primary articular chondrocytes an in vitro study Sophie J Gilbert Emma J Blain Pamela Jones Victor C Duance and Deborah J Mason Connective Tissue Biology Laboratories School of Biosciences Cardiff University Museum Avenue Cardiff Wales UK Corresponding author Sophie J Gilbert gilbertsj1@cardiff.ac.uk Received 23 Mar 2006 Revisions requested 12 Apr 2006 Revisions received 18 Apr 2006 Accepted 20 Apr 2006 Published 12 May 2006 Arthritis Research Therapy 2006 8 R89 doi 10.1186 ar1 961 This article is online at http arthritis-research.com content 8 4 R89 2006 Gilbert et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract We previously established a role for the second messenger ceramide in protein kinase R PKR -mediated articular cartilage degradation. Ceramide is known to play a dual role in collagen gene regulation with the effect of ceramide on collagen promoter activity being dependent on its concentration. Treatment of cells with low doses of sphingomyelinase produces small increases in endogenous ceramide. We investigated whether ceramide influences articular chondrocyte matrix homeostasis and if so the role of PKR in this process. Bovine articular chondrocytes were stimulated for 7 days with sphingomyelinase to increase endogenous levels of ceramide. To inhibit PKR 2-aminopurine was added to duplicate cultures. De novo sulphated glycosaminoglycan and collagen synthesis were measured by adding 35S -sulphate and 3H -proline to the media respectively. Chondrocyte phenotype was investigated using RT-PCR and Western blot analysis. Over 7
