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Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "Walnut (Juglans regia L.) micropropagation. | 149s Ann. Sci. For. 1989 46 suppl. 149s-151s Forest Tree Physiology E. Dreyer et al. eds. Elsevier INRA Walnut Juglans regia L. micropropagation M.A. Revilla J. Majada and R. Rodríguez Lab. Fisiologia Vegetal Universidad de Oviedo Espana Introduction One of the main problems involved in walnut micropropagation by tissue culture techniques is the low rate of multiplication. This can be due to the slow growth of the regenerated shoots leading to extended culture periods and thus resulting in the appearance of latent contamination in the culture Somers et al. 1982 Driver and Kuniyuki 1984 McGranahan eta . 1986 . To overcome this problem we have tried to culture nodal segments from embryonic and juvenile material in a double-phase system which has been shown to increase production of axillary shoots Viseur 1985 and to include in the culture medium antibiotic mixtures to prevent bacterial contamination Phillips et al. 1981 Young etal. 1984 . This research is being conducted to optimize the micropropagation techniques for walnut Juglans regia L Materials and Methods Experiments have been made with embryonic and juvenile nodal segments of walnut. Embryonic axes were excised from seeds previously imbibed for 24 h in water and disinfect ed for 5 min in 0.5 NaCIO solution followed by 5 min in 75 ethanol and 3 rinses in sterile distilled water. Embryonic axes were cultured for 8 wk before excising the nodal segments. Juvenile material was taken from 2-3 mo old plantlets germinated under greenhouse conditions that had been sprayed every 15 d with a solution of 0.04 g l kasugamicin Lainco 0.97 g l zineb Agrocros and 0.38 g l cupric oxychloride Agrocros . Before taking the explants from the juvenile material the plantlets were sprayed 2 or 3 times every 5 d with a solution of 100 mg l BAP benzylaminopurine and 50 mg l GA3 gibberellin McGranahan et al. 1988 to induce vigorous growth. The medium used was MS Murashige and Skoog 1962 supplemented with 30 sucrose 0.7 agar and .
