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Tham khảo luận văn - đề án 'báo cáo khoa học: energetic and metabolic transient response of saccharomyces cerevisiae to benzoic acid', luận văn - báo cáo, báo cáo khoa học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | IFEBS Journal Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid M. T. A. P. Kresnowati W. A. van Winden W. M. van Gulik and J. J. Heijnen Department of Biotechnology Delft University of Technology The Netherlands Keywords adaptation benzoic acid chemostat transient yeast Correspondence J. J. Heijnen Department of Biotechnology Delft University of Technology Julianalaan 67 2628 BC Delft The Netherlands Fax 31 15 278 2355 Tel 31 15 278 2342 E-mail j.j.heijnen@tudelft.nl Present address Microbiology and Bioprocess Technology Laboratory Department of Chemical Engineering Bandung Institute of Technology Indonesia Received 2 January 2008 revised 29 August 2008 accepted 4 September 2008 doi 10.1111 j.1742-4658.2008.06667.x Saccharomyces cerevisiae is known to be able to adapt to the presence of the commonly used food preservative benzoic acid with a large energy expenditure. Some mechanisms for the adaptation process have been suggested but its quantitative energetic and metabolic aspects have rarely been discussed. This study discusses use of the stimulus response approach to quantitatively study the energetic and metabolic aspects of the transient adaptation of S. cerevisiae to a shift in benzoic acid concentration from 0 to 0.8 mM. The information obtained also serves as the basis for further utilization of benzoic acid as a tool for targeted perturbation of the energy system which is important in studying the kinetics and regulation of central carbon metabolism in S. cerevisiae. Using this experimental set-up we found significant fast-transient 3000 s increases in O2 consumption and CO2 production rates of 50 which reflect a high energy requirement for the adaptation process. We also found that with a longer exposure time to benzoic acid S. cerevisiae decreases the cell membrane permeability for this weak acid by a factor of 10 and decreases the cell size to 80 of the initial value. The intracellular metabolite profile in the new .
