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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Lymphocyte Responses to Chymotrypsin- or Trypsin VDigested b-Lactoglobulin in Patients with Cow’s Milk Allergy. | ORIGINAL ARTICLE Lymphocyte Responses to Chymotrypsin- or Trypsin V-Digested b-Lactoglobulin in Patients with Cow s Milk Allergy Masashi Kondo MD PhD Toshiyuki Fukao MD PhD Shinji Shinoda MD PhD Norio Kawamoto MD PhD Hideo Kaneko MD PhD Zenichiro Kato MD PhD Eiko Matsui MD PhD Takahide Teramoto MD PhD Taku Nakano PhD and Naomi Kondo MD PhD Chymotrypsin- or trypsin V- a mixture of trypsin and chymotrypsin digested b-lactoglobulin BLG peptides were prepared and were confirmed to have much less immunoglobulin lg G and lgE reactivity compared with intact BLG by IgG inhibition enzyme-linked immunosorbent assay and IgE dot blotting. The lymphocyte responses to intact BLG and these peptides were examined using peripheral blood mononuclear cells PBMCs from 10 patients with cow s milk allergy. The PBMCs from most patients had lower lymphocyte responses to chymotrypsin- and trypsin V-digested BLG peptides than those to intact BLG. However PBMCs from one and two patients retained significant proliferative responses to both peptides and to only the former peptide respectively. Interferon-c production stimulated by chymotrypsin-digested peptides was still detectable in all five patients tested. Chymotrypsin-digested BLG reduced lgE reactivity but still induced some lymphocyte responses. Cow s milk is one of the most common food allergens in the first year of life with approximately 2 to 2.5 of infants experiencing allergic reactions to it. The majority of children outgrow their allergy to cow s milk before the age of 3 years but 15 of infants with immunoglobulin Ig E-mediated cow s milk allergy retain their sensitivity into the second decade.1 2 The therapy for food allergy is a problem that is still to be resolved. The first therapeutic approach to patients with cow s milk allergy is elimination from the diet of cow s milk proteins. However this is not always easy because cow s milk is an essential constituent of the diet or can be found in other foods as a hidden allergen. .
