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Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Human enterovirus 71 subgenotype B3 lacks coxsackievirus A16-like neurovirulence in mice infection | Virology Journal BioMed Central Research Open Access Human enterovirus 71 subgenotype B3 lacks coxsackievirus AI6-like neurovirulence in mice infection Yoke-Fun Chan1 and Sazaly AbuBakar 2 Address 1Sime Darby Technology Centre 2 Jalan Tandang 46050 Petaling Jaya Selangor Malaysia and 2Department of Medical Microbiology Faculty of Medicine University of Malaya 50603 Kuala Lumpur Malaysia Email Yoke-Fun Chan - yfchan@simenet.com SazalyAbuBakar - sazaly@um.edu.my Corresponding author Published 26 August 2005 Received 22 June 2005 Accepted 26 August 2005 Virology Journal 2005 2 74 doi 10.1186 1743-422X-2-74 This article is available from http www.virologyj.cOm content 2 1 74 2005 Chan and AbuBakar licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background At least three different EV-71 subgenotypes were identified from an outbreak in Malaysia in 1998. The subgenotypes C2 and B4 were associated with the severe and fatal infections whereas the B3 virus was associated with mild to subclinical infections. The B3 virus genome sequences had 85 similarity at the 3 end to CV-A16. This offers opportunities to examine if there are characteristic similarities and differences in virulence between CV-A16 EV-71 B3 and EV-71 B4 and to determine if the presence of the CV-A16-liked genes in EV-71 B3 would also confer the virus with a CV-A16-liked neurovirulence in mice model infection. Results Analysis of human enterovirus 71 EV-71 subgenotype B3 genome sequences revealed that the 3D RNA polymerase and domain Z of the 3 -untranslating region RNA secondary structure had high similarity to CV-A16. Intracerebral inoculation of one-day old mice with the virus resulted in 16 of the mice showing swollen hind limbs and significantly lower .
