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Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài:Knockdown of NPM1 by RNA Interference Inhibits Cells Proliferation and Induces Apoptosis in Leukemic Cell Line. | Int. J. Med. Sci. 2011 8 287 International Journal of Medical Sciences 2011 8 4 287-294 Research Paper Knockdown of NPM1 by RNA Interference Inhibits Cells Proliferation and Induces Apoptosis in Leukemic Cell Line Feng-Xian Qin1 Hui-Yuan Shao1 Xian-Chun Chen1 Shi Tan1 Hui-Juan Zhang1 Zong-Yu Miao1 Li Wang2 Hui-Chen3 Ling Zhang1 H 1. Key Laboratory of Laboratory Medical Diagnostics Ministry of Education Department of Laboratory Medicine Chongqing Medical University Chongqing 400016 China. 2. Department of Hematology the First Affiliated Hospital Chongqing Medical University Chongqing 400016 China. 3. Department of Laboratory Medicine the First Affiliated Hospital Chongqing Medical University Chongqing 400016 China. H Corresponding author Ling Zhang Department of Laboratory Medicine Chongqing Medical University 1 Yixueyuan Road Chongqing 400016 China. Tel 86 023-68485223 Fax 86 023-68485005 Email lingzhang@cqmu.edu.cn Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License http creativecommons.org licenses by-nc-nd 3.0 . Reproduction is permitted for personal noncommercial use provided that the article is in whole unmodified and properly cited. Received 2011.02.21 Accepted 2011.04.11 Published 2011.04.20 Abstract Nucleophosmin NPM1 is an abundant and ubiquitously expressed phosphoprotein that is known to influence solid tumors progression. However little is known about the role of NPM1 in leukemia. Here we knocked down the NPMI expression by RNA interference to investigate the role of NPM 1 in leukemic cells proliferation and apoptosis. The interference vector pNPM1-shRNA was constructed and transfected into the human leukemic K562 cell line. The expression levels of NPMI mRNA and protein were detected by quantitative real-time PCR and Western blot respectively. Cells proliferation potential in vitro was assessed by methyl thiazolyl tetrazolium MTT and colony formation assays. Flow cytometry was .
