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Báo cáo y học: " A Novel Variable Number of Tandem Repeat of the Natriuretic Peptide Precursor B gene’s 5’-Flanking Region is Associated with Essential Hypertension among Japanese Females"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: A Novel Variable Number of Tandem Repeat of the Natriuretic Peptide Precursor B gene’s 5’-Flanking Region is Associated with Essential Hypertension among Japanese Females. | Int. J. Med. Sci. 2007 4 146 Research Paper International Journal of Medical Sciences ISSN 1449-1907 www.medsci.org 2007 4 3 146-152 Ivyspring International Publisher. All rights reserved A Novel Variable Number of Tandem Repeat of the Natriuretic Peptide Precursor B gene s 5 -Flanking Region is Associated with Essential Hypertension among Japanese Females Kotoko Kosuge1 Masayoshi Soma1 3 Tomohiro Nakayama2 Noriko Aoi2 Mikano Sato2 Yoichi Izumi1 Koichi Matsumoto1 1. Division of Nephrology and Endocrinology Department of Medicine Nihon University School of Medicine Tokyo Japan 2. Division of Molecular Diagnostics Advanced Medical Research Center Nihon University School of Medicine Tokyo Japan 3. Division of General Medicine Department of Medicine Nihon University School of Medicine Tokyo Japan Correspondence to Tomohiro Nakayama M.D. Division of Molecular Diagnostics Advanced Medical Research Center Nihon University School of Medicine 30-1 Ooyaguchi-kamimachi Itabashi-ku Tokyo 173-8610 Japan. Tel 81 3-3972-8111 Ext. 8205 Fax 81 3-5375-8076 E-mail tnakayam@med.nihon-u.ac.jp Received 2006.12.04 Accepted 2007.05.15 Published 2007.05.16 Background Brain natriuretic peptide BNP acts primarily as a cardiac hormone it is produced by the ventricle and has both vasodilatory and natriuretic actions. Therefore the BNP gene is thought to be a candidate gene for essential hypertension EH . The present study identified variants in the 5 -flanking region of natriuretic peptide precursor B NPPB gene and assessed the relationship between gene variants and Eh. Methods The polymerase chain reaction-single strand conformation polymorphism method and nucleotide sequencing were used to identify variants. Results A novel variable number of tandem repeat VNTR polymorphism in the 5 -flanking region -1241 nucleotides from the major transcriptional initiation site was discovered. This VNTR polymorphism is a tandem repeat of the 4-nucleotide sequence TTTC. There were 8 alleles ranging from .