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Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Induction of Th1Immune responses following laser ablation in a murine model of colorectal liver metastases | Lin et al. Journal of Translational Medicine 2011 9 83 http www.translational-medicine.eom content 9 1 83 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Induction of Thllmmune responses following laser ablation in a murine model of colorectal liver metastases Wen Xu Lin Theodora Fifis Caterina Malcontenti-Wilson Mehrdad Nikfarjam Vijayaragavan Muralidharan Linh Nguyen and Christopher Christophi Abstract Background Preliminary experimental studies have suggested that the in situ destruction of tumor tissue by local laser ablation LA may also stimulate host immunity against cancer. We investigated local and systemic induction of immune responses after laser ablation in the setting of residual tumor. Methods A murine colorectal cancer CRC liver metastasis model was used. Selected tumors of liver CRC bearing mice and livers of mice without tumor induction were treated with LA. Liver and tumor tissues from the ablation sites and from distant sites were collected at various time points following LA and changes in CD3 T cells and Kupffer cells F4 80 marker infiltration and the expression of interferon gamma IFNy were investigated by immunohistochemistry and ELISpot. Base line levels of CD3 T cells and Kupffer cells were established in untreated mice. Results The presence of tumor induced significant accumulation of CD3 T cells and Kupffer cells at the tumorhost interface within the tumor vascular lakes and increased their baseline concentration within the liver parenchyma. LA of the liver induced accumulation of CD3 T-cells and Kupffer cells at the site of injury and systemic induction of immune responses as discerned by the presence of IFNg secreting splenocytes. LA of liver tumors induced significant increase of CD3 T-cells at site of injury within normal liver parenchyma and the tumor-host interface of both ablated and distant tumors. In contrast Kupffer cells only accumulated in ablated tumors and the liver parenchyma but not in distant tumors. IFNg expression
