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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Ultrasound-targeted microbubble destruction mediated herpes simplex virus-thymidine kinase gene treats hepatoma in mice | Zhou et al. Journal of Experimental Clinical Cancer Research 2010 29 170 http www.jeccr.eom content 29 1 170 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Ultrasound-targeted microbubble destruction mediated herpes simplex virus-thymidine kinase gene treats hepatoma in mice 1 1 f I 1 -I- 1 2 I- z- 1 1 Shiji Zhou Shengwei Li Zuojin Liu Yong Tang Zhigang Wang Jianping Gong Changan Liu Abstract Objective The purpose of the study was to explore the anti-tumor effect of ultrasound -targeted microbubble destruction mediated herpes simplex virus thymidine kinase HSV-TK suicide gene system on mice hepatoma. Methods Forty mice were randomly divided into four groups after the models of subcutaneous transplantation tumors were estabilished 1 PBS 2 HSV-TK 3 HSV-TK ultrasound HSV-TK US 4 HSV-TK ultrasound microbubbles HSV-TK US MB . The TK protein expression in liver cancer was detected by western-blot. Applying TUNEL staining detected tumor cell apoptosis. At last the inhibition rates and survival time of the animals were compared among all groups. Results The TK protein expression of HSV-TK MB US group in tumor-bearing mice tissues were significantly higher than those in other groups. The tumor inhibitory effect of ultrasound-targeted microbubble destruction mediated HSV-TK on mice transplantable tumor was significantly higher than those in other groups p 0.05 and can significantly improve the survival time of tumor-bearing mice. Conclusion Ultrasound-targeted microbubble destruction can effectively transfect HSV-TK gene into target tissues and play a significant inhibition effect on tumors which provides a new strategy for gene therapy in liver cancer. Introduction Hepatocellular carcinoma HCC is one of the malignant tumors with high incidence around the world 1 2 . More than one million new cases appeared each year particularly in the Asia-Pacific region. This disease has rapid progress high recurrence rate and traditional treatments have limited. .
