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Tham khảo tài liệu 'báo cáo hóa học: " discovery of herpesviruses in multi-infected primates using locked nucleic acids (lna) and a bigenic pcr approach"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Open Access Methodology Discovery of herpesviruses in multi-infected primates using locked nucleic acids LNA and a bigenic PCR approach Sandra Prepens1 Karl-Anton Kreuzer2 Fabian Leendertz3 4 Andreas Nitsche3 and Bernhard Ehlers 1 Address 1P14 Molekulare Genetik und Epidemiologie von Herpesviren Robert Koch-Institut Nordufer 20 13353 Berlin Germany 2Klinik I fur Innere Medizin Joseph-Stelzmann-Strafie 9 50924 Koln Germany 3Zentrum fur Biologische Sicherheit Robert Koch-Institut Nordufer 20 13353 Berlin Germany and 4Max-Planck-Institut fur Evolutionare Anthropologie Deutscher Platz 6 04103 Leipzig Germany Email Sandra Prepens - prepenss@rki.de Karl-Anton Kreuzer - karl-anton.kreuzer@uni-koeln.de Fabian Leendertz - leendertzf@rki.de Andreas Nitsche - nitschea@rki.de Bernhard Ehlers - ehlersb@rki.de Corresponding author Published 6 September 2007 Received 20 July 2007 Accepted 6 September 2007 Virology journal 2007 4 84 doi 10.1186 1743-422X-4-84 This article is available from http www.virologyj.cOm content 4 1 84 2007 Prepens et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Targeting the highly conserved herpes DNA polymerase DPOL gene with PCR using panherpes degenerate primers is a powerful tool to universally detect unknown herpesviruses. However vertebrate hosts are often infected with more than one herpesvirus in the same tissue and panherpes DPOL PCR often favors the amplification of one viral sequence at the expense of the others. Here we present two different technical approaches that overcome this obstacle i Pan-herpes DPOL PCR is carried out in the presence of an oligonucleotide substituted .
