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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Induction of apoptosis and inhibition of cell growth by tbx5 knockdown contribute to dysmorphogenesis in Zebrafish embryos. | Lu et al. Journal of Biomedical Science 2011 18 73 http www.jbiomedsci.eom content 18 1 73 BIN sc The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Induction of apoptosis and inhibition of cell growth by tbx5 knockdown contribute to dysmorphogenesis in Zebrafish embryos lonhar I I I1 2 t T 7I I -Fl in Tctỉì2 3 Qiolin f hnn4 Cbi IX 1 I Vob4 Danhinn IGnri1 2 Antanri v nn1 2 I Icin I I I oo5 Jenier Lu izuciiuii Tsai Sielii enuu Shuyu ICII Reibiiy lolly Aiiiaiiy laiy Hsiiyu Lee aid Jennkan Lu4t Abstract Background The tbx5 mutatioi ii humai causes Holt-Oram syidrome ai autosomal domiiait coiditioi characterized by a familial history of coiyeiital heart defects aid preaxlal radial upper-limb defects. We report aberrait apoptosis aid dormait cell growth over head heart truik fii aid tail of zebrafish embryos with tbx5 deficieicy correspoid to the dysmorphoyeiesis of tbx5 morphaits. Methods Wild-type zebrafish embryos at the 1-cell staye were iijected with 4.3 il of 19.4 iy of tbx5 morpholiio or mismatch-tbx5-MO respectively ii tbx5 morphaits aid mismatched coitrol yroup. Semi-quaititative RT-PCR was used to for expressioi aialysis of apoptosis aid cell cycle-related yeies. TUNEL aid immuiohistochemical assay showed the apoptosis spots withii the local tissues. Ultra-structure of cardiac myocardium was examiied by traismissioi electroi microscope. Results Apoptosis-related yeies bad bax aid bcl2 aid cell cycle-related yeies cdk2 pcia p27 aid p57 showed remarkable iicreases ii traiscriptioial level by RT-PCR. Usiiy a TUNEL aid immiuohistochemical assay apoptosis was observed ii the oryais iicludiiy the head heart pectoral fiis truik aid tail of tbx5 kiockdowi embryos. Uider traismissioi electroi microscopic examiiatioi mitochoidria ii cardiomyocytes became swollei aid the myocardium was laryely disoryaiized with a disarrayed appearaice compatible with reduced .
