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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Differential regulation of diacylglycerol kinase isoform in human failing hearts. | Bilim et al. Journal of Cardiothoracic Surgery 2011 6 65 http www.cardiothoracicsurgery.Org content 6 1 65 JCTS JOURNAL OF CARDIOTHORACIC SURGERY RESEARCH ARTICLE Open Access Differential regulation of diacylglycerol kinase isoform in human failing hearts 1 1 2 3 1 1 Olga Bilim Tetsuro Shishido Shuji Toyama Satoshi Suzuki Toshiki Sasaki Tatsuro Kitahara Mitsuaki Sadahiro2 Yasuchika Takeishi3 and Isao Kubota1 Abstract Evidence from several studies indicates the importance of Gaq protein-coupled receptor GPCR signaling pathway which includes diacylglycerol DAG and protein kinase C in the development of heart failure. DAG kinase DGK acts as an endogenous regulator of GPCR signaling pathway by catalyzing and regulating DAG. Expressions of DGK isoforms a E and z in rodent hearts have been detected however the expression and alteration of DGK isoforms in a failing human heart has not yet been examined. In this study we detected mRNA expressions of DGK isoforms g h E and z in failing human heart samples obtained from patients undergoing cardiovascular surgery with cardiopulmonary bypass. Furthermore we investigated modulation of DGK isoform expression in these hearts. We found that expressions of DGKh and DGKZ were increased and decreased respectively whereas those of DGKg and DGKe remained unchanged. This is the first report that describes the differential regulation of DGK isoforms in normal and failing human hearts. Introduction Epidemiological studies have suggested that cardiac hypertrophy is an independent risk factor for the development of heart failure and is associated with increased cardiac morbidity and mortality in patients with cardiovascular diseases 1-3 . Recent in vivo and in vitro studies have focused on protein kinase signaling cascades as the molecular mechanisms regulating the hypertrophic response of cardiomyocytes 4 5 . Among these signaling pathways the Gaq protein-coupled receptor GPCR signaling pathway which includes diacylglycerol DAG and protein
