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Handbook of Microbiological Media, Fourth Edition part 196 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | YI-S Medium 1945 Use For the cultivation and maintenance ofXanthomonas campestris andXanthomonas oryzae. YGLM Yeast Glucose Litmus Milk Composition per liter Glucose.10.0g Skim milk powder.8.0g Yeast extract.3.0g Litmus.0.75g Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly for 15-20 min. Distribute into tubes or flasks. Autoclave for 10 min at 10 psi pressure-115 C. Incubate for 1 week at 30 C to check for sterility before use. Use For the cultivation of Gemella morbillorum. YGLM with Chalk Yeast Glucose Litmus Milk with Chalk Composition per liter CaCO3.20.0g Glucose.10.0g Yeast extract.3.0g Litmus.0.75g Skim milk.100.0mL Preparation of Medium Add components except skim milk to distilled deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Add 10.0mL of skim milk. Distribute into tubes. Autoclave for 10 min at 10 psi pressure-115 C. Incubate for 1 week at 30 C to check for sterility before use. Use For the cultivation of Gemella morbillorum. YGLPB Medium Composition per liter Peptone.10.0g Lab-Lemco.8.0g Glucose.5.0g Lactose.5.0g Yeast extract.3.0g KH2PO4.2.5g K2HPO4.2.5g MgSO4-7H2O.0.2g MnSO4-4H2O.0.05g Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure-121 C. Use For the cultivation of Lactobacillus delbrueckii. YGLPB Medium Composition per liter Peptone.10.0g Beef extract.8.0g Glucose.5.0g Lactose.5.0g Yeast extract.3.0g K2HPO4.2.5g KH2PO4.2.5g MgSO4-7H2O.0.2g MnSO4-4H2O.0.05g pH 6.8 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Carnobacterium divergens Carnobacterium piscícola Lactobacillus delbrueckii .
