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Handbook of Microbiological Media, Fourth Edition part 50 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | DCLSHiVegAgar Hajna 485 Preparation of Glucose Solution Add glucose to distilled deion-ized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except glucose solution to distilled deionized water and bring volume to 980.0mL. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add 20.0mL of sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and maintenance of Escherichia coli. DCLS Agar Deoxycholate Citrate Lactose Sucrose Agar Composition per liter Agar.12.0g Sodium citrate-3H2O.10.5g Lactose.5.0g Na2S2O3.5.0g Sucrose. 5.0g Pancreatic digest of casein.3.5g Peptic digest of animal tissue.3.5g Beef extract.3.0g Sodium deoxycholate.2.5g Neutral Red.0.03g pH 7.2 0.1 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Do not overheat. Do not autoclave. Pour into sterile Petri dishes in 20.0mL volumes. Use For the selective isolation of Salmonella species Shigella species and Vibrio species from fecal specimens. DCLS Agar Composition per liter Agar.12.0g Sodium citrate.10.0g Proteose peptone.7.0g Lactose.5.0g Na2S2O3.5.0g Sucrose. 5.0g Beef extract.3.0g Sodium deoxycholate.2.5g Neutral Red.0.03 pH 7.2 0.1 at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Do not overheat. Do not autoclave. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the selective isolation of Salmonella species Shigella species and Vibrio species from fecal specimens. DCLS Agar Hajna Composition per liter .
