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Handbook of Microbiological Media, Fourth Edition part 195 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Yeast Malt Extract Catalase Agar 1935 may be rendered selective by adjusting the pH to 3.0-4.0 at 45 -55 C or by the addition of antibiotics at 45 -50 C or below. Use For the cultivation and maintenance of Dekkera abstinens. Yeast Malt Extract Broth with Glucose YM Broth with Glucose Composition per liter Glucose.290.0g Peptone.5.0g CaCO3.5.0g Yeast extract.3.0g Malt extract.3.0g pH 6.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. The medium may be rendered selective by adjusting the pH to 3.0-4.0 at 45 -55 C or by the addition of antibiotics . Use For the cultivation of yeasts and filamentous fungi. Yeast Malt Extract Broth with 1.0 Methanol YM Broth with 1.0 Methanol Composition per liter Glucose.10.0g Peptone.5.0g Yeast extract.3.0g Malt extract.3.0g Methanol.10.0mL pH 6.2 0.2 at 25 C Preparation of Medium Add components except methanol to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Filter sterilize the methanol. Aseptically add the sterile methanol to the cooled sterile basal medium. Mix thoroughly. Distribute into sterile flasks or tubes. Use For the cultivation and maintenance of Pichia angusta and Wick-erhamiella domercqiae. Yeast Malt Extract Broth with 18 Sodium Chloride YM Broth with 18 NaCl Composition per liter NaCl.180.0g Glucose.10.0g Peptone.5.0g Yeast extract.3.0g Malt extract.3.0g pH 6.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Zygosaccharomyces rouxii. Yeast Malt Extract Broth with 40.0 Sucrose YM Broth with 40.0 Sucrose Composition per liter Sucrose.400.0g Glucose.10.0g Peptone.5.0g Yeast .
