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Handbook of Microbiological Media, Fourth Edition part 190 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Vibrio vulnificus Agar 1885 Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of Vibrio natriegens. Vibrio parahaemolyticus Agar VP Agar Composition per liter Agar.20.0g NaCl.20.0g Sucrose.20.0g Sodium citrate.10.0g Na2S2O3-5H2O.10.0g Peptone.10.0g Sodium taurocholate.5.0g Yeast extract.5.0g Sodium lauryl sulfate.0.2g Bromthymol Blue.0.04g Thymol Blue.0.04g pH 8.6 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave. Pour into sterile Petri dishes. Use For the isolation cultivation enumeration and presumptive identification of coliforms in milk food and other specimens of sanitary significance. For the enumeration of bacteria in cheese especially Pseudomonas fragi Pseudomonas viscosa and Alcaligenes metalcaligenes. Sucrose-fermenting bacteria appear as yellow colonies with pale yellow peripheries. Sucrose-nonfermenting bacteria appear as mucoid green colonies with a dark green center. Vibrio parahaemolyticus Sucrose Agar VPSA Composition per liter NaCl.30.0g Agar.15.0g Sucrose.10.0g Yeast extract.7.0g Tryptose.5.0g Pancreatic digest of casein.5.0g Bile salts No. 3.1.5g Bromthymol Blue.0.025g pH 8.6 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave. Cool to 50 C. Pour into sterile Petri dishes in 20.0mL volumes. Allow plates to dry before using. Use For the isolation cultivation and differentiation of Vibrio para-haemolyticus from seafood. Vibrio parahaemolyticus and Vibrio vulnificus appear as blue to green colonies. Other Vibrio species appear as yellow colonies. Vibrio
