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Handbook of Microbiological Media, Fourth Edition part 189 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Ustilago Medium 1875 Preparation of Trace Elements Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Salt Solution Add components to distilled deion-ized water and bring volume to 1.0L. Mix thoroughly. Vitamin Solution Composition per liter Inositol.1.0g Calcium pantothenate.0.2g Choline chloride.0.2g Nicotinic acid.0.2g Thiamine.100.0mg p-Aminobenzoic acid.50.0mg Pyridoxine.50.0mg Riboflavin.50.0mg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Hydrolyzed Nucleic Acids Solution Composition per 80.0mL DNA calf thymus.2.0g RNA.2.0g HCl 1M solution .30.0mL NaOH 1M solution .30.0mL Preparation of Hydrolyzed Nucleic Acids Solution Add DNA to 30.0mL of 1M NaOH solution. Add RNA to 30.0mL of 1M HCl solution. Autoclave the two solutions separately for 10 min at 15 psi pres-sure-121 C. Mix the two solutions. Adjust the pH to 6.0. Centrifuge at 5000 x g for 10 min. Decant supernatant solution and filter. Bring volume to 80.0mL with distilled deionized water. Store at -20 C. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of Ustilago species. Ustilago Complete Broth II Composition per liter Glucose.10.0g Hydrolyzed casein.2.5g NH4NO3.1.5g Yeast extract.1.0g Salt solution.62.5mL Vitamin solution.10.0mL Hydrolyzed nucleic acids solution.5.0mL pH 7.0 0.2 at 25 C Salt Solution Composition per liter KH2PO4.16.0g KCl.8.0g Na2SO4.4.0g MgSO4.2.0g CaCl2.1.0g Trace elements solution.8.0mL Trace Elements Solution Composition per liter CuSO4-5H2O.0.4g ZnCl2.0.4g MnCl24H2O.0.14g FeCl3-6H2O.100.0mg H3BO3.60.0mg Na2MoO4-2H2O.40.0mg Preparation of Trace Elements Solution Add components
