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Handbook of Microbiological Media, Fourth Edition part 186 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Tryptone Yeast Extract Agar 1 1845 MnCl2-4H2O.2.0mg Glucose solution.50.0mL pH 7.5 0.2 at 25 C Glucose Solution Composition per 50.0mL Glucose.20.0g Preparation of Glucose Solution Add glucose to distilled deion-ized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except glucose solution to distilled deionized water and bring volume to 950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add 50.0mL of sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Clostridium butyricum and Clostridium roseum. Tryptone Water Broth Tryptone Broth Composition per liter Pancreatic digest of casein.10.0g NaCl.5.0g pH 7.5 0.2 at 25 C Source This medium is available as a premixed powder from Oxoid Unipath. Preparation of Medium Dissolve 15.0g in 1.0L of distilled water and distribute into final containers. Sterilize by autoclaving at 121 C for 15 min. Use For the cultivation of production of indole by microorganisms. Tryptone Water HiVeg Tryptone Broth HiVeg Composition per liter Plant hydrolysate.20.0g NaCl.5.0g pH 7.0 0.2 at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to tap water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For production of indole by microorganisms. Tryptone Water Broth HiVeg Composition per liter Plant hydrolysate.10.0g Glucose.5.0g K2HPO4.1.25g Yeast extract.1.0g Bromcresol Purple.0.04g pH 7.3 0.2 at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to tap water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Dis tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Salmonella
